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Protein Ontology report - NS12345
PR:000036838 - http://purl.obolibrary.org/obo/PR_000036838
 
  Protein Forms  Complex  Annotations      
Ontology Information Show OBO stanza
  PRO ID
PR:000036838   
  PRO nameNS12345 
  Synonyms
PRO-Short-label: EXACT:DenV-NS12345
Other: EXACT:Non-structural proteins | Nonstructural proteins | NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5
  Definition"A dengue virus genome polyprotein proteolytic cleavage product that is derived from dengue virus genome polyprotein, N-glycosylated form and that contains the entire non-structural region of the dengue virus genome polyprotein only and is responsible for directing viral genomic RNA replication, including synthesizing negative- and positive-strand RNAs and forming the viral RNA cap structure. It consists of NS1, NS2A, NS3, NS4A, 2K, NS4B, and NS5." [PMID:1433530, PMID:22022449, PRO:ES] 
  CommentRequested by=IDODEN. 
  PRO Categorymodification 
  ParentPR:000036818 dengue virus genome polyprotein proteolytic cleavage product
PR:000036817 dengue virus genome polyprotein, N-glycosylated form
  Terms by PRO Category
Organism-Independent Organism-Specific
         Category          Number of Terms          Category          Number of Terms
         Modification17                                                  Organism-Modification 8                                        
  Term Hierarchy
  Visualization
DAG: OLS:
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Protein Forms Switch to Compact View   

Category  

PRO ID

Name

Short Name

Definition

Ann. Has Annotation?

Comp. In Complex?
modification



PR:000036838

NS12345

DenV-NS12345


"A dengue virus genome polyprotein proteolytic cleavage product that is derived from dengue virus genome polyprotein, N-glycosylated form and that contains the entire non-structural region of the dengue virus genome polyprotein only and is responsible for directing viral genomic RNA replication, including synthesizing negative- and positive-strand RNAs and forming the viral RNA cap structure. It consists of NS1, NS2A, NS3, NS4A, 2K, NS4B, and NS5." [PMID:1433530, PMID:22022449, PRO:ES]


modification



PR:000036826

NS2A-alpha

DenV-NS2Aalpha


"A dengue virus genome polyprotein proteolytic cleavage product that consists of 188 amino acids at the N-terminal end of NS2A." [PMID:23408612, PRO:ES]


modification



PR:000036827

NS2B

DenV-NS2B


"A dengue virus genome polyprotein proteolytic cleavage product that is a non-structural protein encoded by the dengue virus RNA genome containing a conserved hydrophilic domain necessary for protease activity in cleavage of the dengue virus genome polyprotein, N-glycosylated form." [PMID:10744671, PRO:ES]


Yes
organism-modification



PR:000044761

NS2B (Dengue virus 2 Thailand/NGS-C/1944)

NS2B (DEN2N)


"An NS2B in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 1346-1475." [PRO:DNx]

Yes

modification



PR:000036839

NS345

DenV-NS345


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS12345 by the two-component proteinase NS2B-NS3 at the NS2B-NS3 site consisting of NS3, NS4A, NS4B, and NS5." [PMID:1433530, PRO:ES]


modification



PR:000036828

NS3

DenV-NS3


"A dengue virus genome polyprotein proteolytic cleavage product that is a non-structural, multifunctional protein encoded by the dengue virus RNA genome with a serine protease domain." [PMID:10744671, PRO:ES]


Yes
organism-modification



PR:000044762

NS3 (Dengue virus 2 Thailand/NGS-C/1944)

NS3 (DEN2N)


"An NS3 in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 1476-2093." [PRO:DNx]

Yes

modification



PR:000036840

NS4A-NS4B-NS5

DenV-NS4AB5


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS345 by the two-component proteinase NS2B-NS3 at the NS3-NS4A site consisting of NS4A, 2K, NS4B, and NS5." [PMID:1531368, PRO:ES]


modification



PR:000036841

NS4AB

DenV-NS4AB


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS4A-NS4B-NS5 by the two-component proteinase NS2B-NS3 at the NS4B-NS5 sites consisting of NS4A, 2K, and NS4B." [PMID:1531368, PRO:ES]


modification



PR:000036830

NS4A

DenV-NS4A


"A dengue virus genome polyprotein proteolytic cleavage product that is a highly hydrophobic non-structural protein encoded by the dengue virus RNA genome that associates with membranes via 4 internal hydrophobic regions which are all able to mediate membrane targeting of a cytosolic reporter protein." [PMID:17276984, PRO:ES]


organism-modification



PR:000044763

NS4A (Dengue virus 2 Thailand/NGS-C/1944)

NS4A (DEN2N)


"An NS4A in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 2094-2220." [PRO:DNx]

Yes

organism-modification



PR:000044764

NS4A (Dengue virus 2 Thailand/16681/84)

NS4A (DEN26)


"An NS4A in Dengue virus 2 Thailand/16681/84. UniProtKB:P29990, 2094-2220." [PRO:DNx]

Yes

modification



PR:000036842

2KNS4B

DenV-2KNS4B


"A dengue virus genome polyprotein proteolytic cleavage product that assists in the processing of NS4AB consisting of 2K and NS4B." [PMID:21810535, PRO:ES]


modification



PR:000036831

2K

DenV-2K


"A dengue virus genome polyprotein proteolytic cleavage product that is the C-terminal region of NS4A serving as a signal sequence for the translocation of the adjacent NS4B into the endoplasmic reticulum (ER) lumen." [PMID:17276984, PRO:ES]


organism-modification



PR:000044765

2K (Dengue virus 2 Thailand/NGS-C/1944)

2K (DEN2N)


"A 2K in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 2221-2243." [PRO:DNx]

Yes

modification



PR:000036843

NS4B-NS5

DenV-NS4B5


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS4A-NS4B-NS5 at the NS4A-NS4B site consisting of NS4B and NS5, which is believed to be mediated by a host cell signal peptidase." [PMID:1531368, PRO:ES]


modification



PR:000036873

NS2B-NS3 polyprotein precursor

DenV-NS2B3


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS12345 by the two-component protease NS2B-NS3 at the NS2A-NS2B and NS3-NS4 sites and consists of NS2B and NS3." [PMID:10744671, PRO:ES]


modification



PR:000036998

NS1-NS2A

DenV-NS12A


"A dengue virus genome polyprotein proteolytic cleavage product that is cleaved from NS12345 consisting of NS1 and NS2A. It includes two N-glycosylated residues at Asn-130 and Asn-207. Example: UniProtKB:P29990, 776-1345, Asn-905/Asn-982, MOD:00160." [PMID:15592895, PRO:DAN, PRO:ES]


modification



PR:000036824

NS1

DenV-NS1


"A dengue virus genome polyprotein proteolytic cleavage product that is the N-terminal most non-structural protein within the dengue virus genome polyprotein. Example: UniProtKB:P29990, 776-1127, Asn-905/Asn-982, MOD:00160." [PRO:ES]


Yes
modification



PR:000037000

NS1, with complex sugar attached

DenV-NS1/sugar(+)


"An NS1 that has had the glycan attached to the Asn-130 site modified to a complex sugar." [PRO:ES]


Yes
modification



PR:000037001

NS1, without complex sugar attached

DenV-NS1/sugar(-)


"An NS1 that has not had the glycan attached to the Asn-130 site modified to a complex sugar." [PRO:ES]


Yes
organism-modification



PR:000044758

NS1 (Dengue virus 2 Thailand/NGS-C/1944)

NS1 (DEN2N)


"An NS1 in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 776-1127, Asn-905/Asn-982, MOD:00160." [PRO:DNx]

Yes

modification



PR:000036825

NS2A

DenV-NS2A


"A dengue virus genome polyprotein proteolytic cleavage product that is a non-structural protein encoded by the dengue virus RNA genome and a component of the viral replication complex." [PMID:23408612, PRO:ES]


organism-modification



PR:000044759

NS2A (Dengue virus 2 Thailand/NGS-C/1944)

NS2A (DEN2N)


"An NS2A in Dengue virus 2 Thailand/NGS-C/1944. UniProtKB:P14340, 1128-1345." [PRO:DNx]

Yes

organism-modification



PR:000044760

NS2A (Dengue virus 2 Thailand/16681/84)

NS2A (DEN26)


"An NS2A in Dengue virus 2 Thailand/16681/84. UniProtKB:P29990, 1128-1345." [PRO:DNx]

Yes

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DenV-NS12345 forms found in complexes   

DenV-NS12345 Component
Complexes
PR:000036824 DenV-NS1 PR:000037002 NS1 dimer
PR:000036827 DenV-NS2B PR:000036829 NS2B-NS3 complex
PR:000036828 DenV-NS3 PR:000036829 NS2B-NS3 complex
PR:000037000 DenV-NS1/sugar(+) PR:000037003 NS1 dimer, with complex sugar attached
PR:000037005 NS1 hexamer, with complex sugar attached
PR:000037001 DenV-NS1/sugar(-) PR:000037004 NS1 dimer, without complex sugar unattached
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Functional Annotation (PRO-centric view) Switch to GO Centric View   
PRO Term GO Annotation Evidence
PR:000044758 NS1 (DEN2N)
UniProtKB:P14340, 776-1127, Asn-905/Asn-982, MOD:00160
acts_upstream_of_or_withinGO:0019079 viral genome replication PMID:23408612
GO:0097281 immune complex formation PMID:23408612
located_inGO:0019034 viral replication complex PMID:23408612
PR:000044759 NS2A (DEN2N)
UniProtKB:P14340, 1128-1345
acts_upstream_of_or_withinGO:0019068 virion assembly PMID:23408612
GO:0019076 viral release from host cell PMID:23408612
GO:0019079 viral genome replication PMID:23408612
located_inGO:0019034 viral replication complex PMID:23408612
PR:000044760 NS2A (DEN26)
UniProtKB:P29990, 1128-1345
acts_upstream_of_or_withinGO:0060339 negative regulation of type I interferon-mediated signaling pathway PMID:14612562
PR:000044761 NS2B (DEN2N)
UniProtKB:P14340, 1346-1475
acts_upstream_of_or_withinGO:1900005 positive regulation of serine-type endopeptidase activity PMID:10744671
enablescontributes_to GO:0008236 serine-type peptidase activity PMID:10744671
PR:000044762 NS3 (DEN2N)
UniProtKB:P14340, 1476-2093
acts_upstream_of_or_withinGO:0019082 viral protein processing PMID:10744671
enablesGO:0003724 RNA helicase activity PMID:10744671
GO:0008236 serine-type peptidase activity PMID:10744671
GO:0017111 ribonucleoside triphosphate phosphatase activity PMID:10744671
PR:000044763 NS4A (DEN2N)
UniProtKB:P14340, 2094-2220
acts_upstream_of_or_withinGO:0019079 viral genome replication PMID:17276984
located_inGO:0019034 viral replication complex PMID:17276984
PR:000044764 NS4A (DEN26)
UniProtKB:P29990, 2094-2220
acts_upstream_of_or_withinGO:0060339 negative regulation of type I interferon-mediated signaling pathway PMID:14612562
PR:000044765 2K (DEN2N)
UniProtKB:P14340, 2221-2243
acts_upstream_of_or_withinGO:0045051 protein insertion into ER membrane by internal uncleaved signal-anchor sequence PMID:17276984
enablesGO:0043495 protein-membrane adaptor activity PMID:21810535
located_inGO:0016020 membrane PMID:21810535